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fluidigm sciences 201192b  (fluidigm)


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    fluidigm fluidigm sciences 201192b
    Fluidigm Sciences 201192b, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intercalator+ir+fluidigm/Cell-ID+Intercalator-Ir/pmc13044534-113-13-13
    Average 96 stars, based on 216 article reviews
    fluidigm sciences 201192b - by Bioz Stars, 2026-09
    96/100 stars

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    Labeling:

    Article Title: Human Immune System Variation during 1 Year.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Benzonase Sigma-Aldrich Cat# E1014 1X PBS Rockland Cat# MB-008 EDTA Rockland Cat# MB-014 Sodium Azide Sigma-Aldrich Cat# 71289 Bovine Serum Albumin Sigma-Aldrich Cat# A3059 Paraformaldehyde Polysciences Cat# 00380-1 Intercalator-Ir Fluidigm Cat# 201192B EQ Four Element Calibration Beads Fluidigm Cat# 201078 Maxpar X8 Multimetal labeling kit Fluidigm Cat# 201300 Metal isotopes as chloride salts Fluidigm N/A Dimethyl Sulfoxide Sigma-Aldrich Cat# D8418 Cell-ID Cisplatin Fluidigm Cat# 201064 Metal isotopes as chloride salts Trace Sciences International N/A Protein Stabilizer PBS Candor Bioscience GmbH Cat# 131125 Critical Commercial Assays Test tube with 35 mm nylon mesh Corning Cat# 352235 Cardiometabolic panel Olink AB N/A Cell Regulation panel Olink AB N/A Cardiovascular II panel (CVD II) Olink AB N/A Cardiovascular III panel (CVD III) Olink AB N/A Development panel Olink AB N/A Immune Response panel Olink AB N/A Immuno-Oncology panel Olink AB N/A Oncology II panel Olink AB N/A Inflammation panel Olink AB N/A Metabolism panel Olink AB N/A Neurology, and Organ Damage panel Olink AB N/A Deposited Data FCS files, Mass cytometry This paper Protein expression data This paper Software and Algorithms Mass Cytometry Normalizer (Finck et al., 2013) https://github.com/nolanlab/bead-normalization/releases CyTOF software (v. 6.0.626) - https://www.fluidigm.com/ robCompositions 2.0.5 (Templ et al., 2011) https://cran.r-project.org/web/packages/robCompositions/ index.html Kohonen 3.0.2 (Wehrens and Buydens, 2007) https://cran.r-project.org/web/packages/kohonen/index.html dmbvs (Wadsworth et al., 2017) https://github.com/duncanwadsworth/dmbvs Princurve 2.1.3. (Hastie and Stuetzle, 1989) https://cran.r-project.org/web/packages/princurve/index.html MASS 7.3-51.1 (Venables and Ripley, 2002) https://cran.r-project.org/web/packages/MASS/index.html Sva 3.18.0 N/A http://bioconductor.org/packages/release/bioc/html/sva.html .. REAGENT or RESOURCE SOURCE IDENTIFIER Benzonase Sigma-Aldrich Cat# E1014 1X PBS Rockland Cat# MB-008 EDTA Rockland Cat# MB-014 Sodium Azide Sigma-Aldrich Cat# 71289 Bovine Serum Albumin Sigma-Aldrich Cat# A3059 Paraformaldehyde Polysciences Cat# 00380-1 Intercalator-Ir Fluidigm Cat# 201192B EQ Four Element Calibration Beads Fluidigm Cat# 201078 Maxpar X8 Multimetal labeling kit Fluidigm Cat# 201300 Metal isotopes as chloride salts Fluidigm N/A Dimethyl Sulfoxide Sigma-Aldrich Cat# D8418 Cell-ID Cisplatin Fluidigm Cat# 201064 Metal isotopes as chloride salts Trace Sciences International N/A Protein Stabilizer PBS Candor Bioscience GmbH Cat# 131125 Critical Commercial Assays Test tube with 35 mm nylon mesh Corning Cat# 352235 Cardiometabolic panel Olink AB N/A Cell Regulation panel Olink AB N/A Cardiovascular II panel (CVD II) Olink AB N/A Cardiovascular III panel (CVD III) Olink AB N/A Development panel Olink AB N/A Immune Response panel Olink AB N/A Immuno-Oncology panel Olink AB N/A Oncology II panel Olink AB N/A Inflammation panel Olink AB N/A Metabolism panel Olink AB N/A Neurology, and Organ Damage panel Olink AB N/A Deposited Data FCS files, Mass cytometry This paper Protein expression data This paper Software and Algorithms Mass Cytometry Normalizer (Finck et al., 2013) https://github.com/nolanlab/bead-normalization/releases CyTOF software (v. 6.0.626) - https://www.fluidigm.com/ robCompositions 2.0.5 (Templ et al., 2011) https://cran.r-project.org/web/packages/robCompositions/ index.html Kohonen 3.0.2 (Wehrens and Buydens, 2007) https://cran.r-project.org/web/packages/kohonen/index.html dmbvs (Wadsworth et al., 2017) https://github.com/duncanwadsworth/dmbvs Princurve 2.1.3. (Hastie and Stuetzle, 1989) https://cran.r-project.org/web/packages/princurve/index.html MASS 7.3-51.1 (Venables and Ripley, 2002) https://cran.r-project.org/web/packages/MASS/index.html Sva 3.18.0 N/A http://bioconductor.org/packages/release/bioc/html/sva.html ..

    Mass Cytometry:

    Article Title: Human Immune System Variation during 1 Year.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Benzonase Sigma-Aldrich Cat# E1014 1X PBS Rockland Cat# MB-008 EDTA Rockland Cat# MB-014 Sodium Azide Sigma-Aldrich Cat# 71289 Bovine Serum Albumin Sigma-Aldrich Cat# A3059 Paraformaldehyde Polysciences Cat# 00380-1 Intercalator-Ir Fluidigm Cat# 201192B EQ Four Element Calibration Beads Fluidigm Cat# 201078 Maxpar X8 Multimetal labeling kit Fluidigm Cat# 201300 Metal isotopes as chloride salts Fluidigm N/A Dimethyl Sulfoxide Sigma-Aldrich Cat# D8418 Cell-ID Cisplatin Fluidigm Cat# 201064 Metal isotopes as chloride salts Trace Sciences International N/A Protein Stabilizer PBS Candor Bioscience GmbH Cat# 131125 Critical Commercial Assays Test tube with 35 mm nylon mesh Corning Cat# 352235 Cardiometabolic panel Olink AB N/A Cell Regulation panel Olink AB N/A Cardiovascular II panel (CVD II) Olink AB N/A Cardiovascular III panel (CVD III) Olink AB N/A Development panel Olink AB N/A Immune Response panel Olink AB N/A Immuno-Oncology panel Olink AB N/A Oncology II panel Olink AB N/A Inflammation panel Olink AB N/A Metabolism panel Olink AB N/A Neurology, and Organ Damage panel Olink AB N/A Deposited Data FCS files, Mass cytometry This paper Protein expression data This paper Software and Algorithms Mass Cytometry Normalizer (Finck et al., 2013) https://github.com/nolanlab/bead-normalization/releases CyTOF software (v. 6.0.626) - https://www.fluidigm.com/ robCompositions 2.0.5 (Templ et al., 2011) https://cran.r-project.org/web/packages/robCompositions/ index.html Kohonen 3.0.2 (Wehrens and Buydens, 2007) https://cran.r-project.org/web/packages/kohonen/index.html dmbvs (Wadsworth et al., 2017) https://github.com/duncanwadsworth/dmbvs Princurve 2.1.3. (Hastie and Stuetzle, 1989) https://cran.r-project.org/web/packages/princurve/index.html MASS 7.3-51.1 (Venables and Ripley, 2002) https://cran.r-project.org/web/packages/MASS/index.html Sva 3.18.0 N/A http://bioconductor.org/packages/release/bioc/html/sva.html .. REAGENT or RESOURCE SOURCE IDENTIFIER Benzonase Sigma-Aldrich Cat# E1014 1X PBS Rockland Cat# MB-008 EDTA Rockland Cat# MB-014 Sodium Azide Sigma-Aldrich Cat# 71289 Bovine Serum Albumin Sigma-Aldrich Cat# A3059 Paraformaldehyde Polysciences Cat# 00380-1 Intercalator-Ir Fluidigm Cat# 201192B EQ Four Element Calibration Beads Fluidigm Cat# 201078 Maxpar X8 Multimetal labeling kit Fluidigm Cat# 201300 Metal isotopes as chloride salts Fluidigm N/A Dimethyl Sulfoxide Sigma-Aldrich Cat# D8418 Cell-ID Cisplatin Fluidigm Cat# 201064 Metal isotopes as chloride salts Trace Sciences International N/A Protein Stabilizer PBS Candor Bioscience GmbH Cat# 131125 Critical Commercial Assays Test tube with 35 mm nylon mesh Corning Cat# 352235 Cardiometabolic panel Olink AB N/A Cell Regulation panel Olink AB N/A Cardiovascular II panel (CVD II) Olink AB N/A Cardiovascular III panel (CVD III) Olink AB N/A Development panel Olink AB N/A Immune Response panel Olink AB N/A Immuno-Oncology panel Olink AB N/A Oncology II panel Olink AB N/A Inflammation panel Olink AB N/A Metabolism panel Olink AB N/A Neurology, and Organ Damage panel Olink AB N/A Deposited Data FCS files, Mass cytometry This paper Protein expression data This paper Software and Algorithms Mass Cytometry Normalizer (Finck et al., 2013) https://github.com/nolanlab/bead-normalization/releases CyTOF software (v. 6.0.626) - https://www.fluidigm.com/ robCompositions 2.0.5 (Templ et al., 2011) https://cran.r-project.org/web/packages/robCompositions/ index.html Kohonen 3.0.2 (Wehrens and Buydens, 2007) https://cran.r-project.org/web/packages/kohonen/index.html dmbvs (Wadsworth et al., 2017) https://github.com/duncanwadsworth/dmbvs Princurve 2.1.3. (Hastie and Stuetzle, 1989) https://cran.r-project.org/web/packages/princurve/index.html MASS 7.3-51.1 (Venables and Ripley, 2002) https://cran.r-project.org/web/packages/MASS/index.html Sva 3.18.0 N/A http://bioconductor.org/packages/release/bioc/html/sva.html ..

    Expressing:

    Article Title: Human Immune System Variation during 1 Year.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Benzonase Sigma-Aldrich Cat# E1014 1X PBS Rockland Cat# MB-008 EDTA Rockland Cat# MB-014 Sodium Azide Sigma-Aldrich Cat# 71289 Bovine Serum Albumin Sigma-Aldrich Cat# A3059 Paraformaldehyde Polysciences Cat# 00380-1 Intercalator-Ir Fluidigm Cat# 201192B EQ Four Element Calibration Beads Fluidigm Cat# 201078 Maxpar X8 Multimetal labeling kit Fluidigm Cat# 201300 Metal isotopes as chloride salts Fluidigm N/A Dimethyl Sulfoxide Sigma-Aldrich Cat# D8418 Cell-ID Cisplatin Fluidigm Cat# 201064 Metal isotopes as chloride salts Trace Sciences International N/A Protein Stabilizer PBS Candor Bioscience GmbH Cat# 131125 Critical Commercial Assays Test tube with 35 mm nylon mesh Corning Cat# 352235 Cardiometabolic panel Olink AB N/A Cell Regulation panel Olink AB N/A Cardiovascular II panel (CVD II) Olink AB N/A Cardiovascular III panel (CVD III) Olink AB N/A Development panel Olink AB N/A Immune Response panel Olink AB N/A Immuno-Oncology panel Olink AB N/A Oncology II panel Olink AB N/A Inflammation panel Olink AB N/A Metabolism panel Olink AB N/A Neurology, and Organ Damage panel Olink AB N/A Deposited Data FCS files, Mass cytometry This paper Protein expression data This paper Software and Algorithms Mass Cytometry Normalizer (Finck et al., 2013) https://github.com/nolanlab/bead-normalization/releases CyTOF software (v. 6.0.626) - https://www.fluidigm.com/ robCompositions 2.0.5 (Templ et al., 2011) https://cran.r-project.org/web/packages/robCompositions/ index.html Kohonen 3.0.2 (Wehrens and Buydens, 2007) https://cran.r-project.org/web/packages/kohonen/index.html dmbvs (Wadsworth et al., 2017) https://github.com/duncanwadsworth/dmbvs Princurve 2.1.3. (Hastie and Stuetzle, 1989) https://cran.r-project.org/web/packages/princurve/index.html MASS 7.3-51.1 (Venables and Ripley, 2002) https://cran.r-project.org/web/packages/MASS/index.html Sva 3.18.0 N/A http://bioconductor.org/packages/release/bioc/html/sva.html .. REAGENT or RESOURCE SOURCE IDENTIFIER Benzonase Sigma-Aldrich Cat# E1014 1X PBS Rockland Cat# MB-008 EDTA Rockland Cat# MB-014 Sodium Azide Sigma-Aldrich Cat# 71289 Bovine Serum Albumin Sigma-Aldrich Cat# A3059 Paraformaldehyde Polysciences Cat# 00380-1 Intercalator-Ir Fluidigm Cat# 201192B EQ Four Element Calibration Beads Fluidigm Cat# 201078 Maxpar X8 Multimetal labeling kit Fluidigm Cat# 201300 Metal isotopes as chloride salts Fluidigm N/A Dimethyl Sulfoxide Sigma-Aldrich Cat# D8418 Cell-ID Cisplatin Fluidigm Cat# 201064 Metal isotopes as chloride salts Trace Sciences International N/A Protein Stabilizer PBS Candor Bioscience GmbH Cat# 131125 Critical Commercial Assays Test tube with 35 mm nylon mesh Corning Cat# 352235 Cardiometabolic panel Olink AB N/A Cell Regulation panel Olink AB N/A Cardiovascular II panel (CVD II) Olink AB N/A Cardiovascular III panel (CVD III) Olink AB N/A Development panel Olink AB N/A Immune Response panel Olink AB N/A Immuno-Oncology panel Olink AB N/A Oncology II panel Olink AB N/A Inflammation panel Olink AB N/A Metabolism panel Olink AB N/A Neurology, and Organ Damage panel Olink AB N/A Deposited Data FCS files, Mass cytometry This paper Protein expression data This paper Software and Algorithms Mass Cytometry Normalizer (Finck et al., 2013) https://github.com/nolanlab/bead-normalization/releases CyTOF software (v. 6.0.626) - https://www.fluidigm.com/ robCompositions 2.0.5 (Templ et al., 2011) https://cran.r-project.org/web/packages/robCompositions/ index.html Kohonen 3.0.2 (Wehrens and Buydens, 2007) https://cran.r-project.org/web/packages/kohonen/index.html dmbvs (Wadsworth et al., 2017) https://github.com/duncanwadsworth/dmbvs Princurve 2.1.3. (Hastie and Stuetzle, 1989) https://cran.r-project.org/web/packages/princurve/index.html MASS 7.3-51.1 (Venables and Ripley, 2002) https://cran.r-project.org/web/packages/MASS/index.html Sva 3.18.0 N/A http://bioconductor.org/packages/release/bioc/html/sva.html ..

    Software:

    Article Title: Human Immune System Variation during 1 Year.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Benzonase Sigma-Aldrich Cat# E1014 1X PBS Rockland Cat# MB-008 EDTA Rockland Cat# MB-014 Sodium Azide Sigma-Aldrich Cat# 71289 Bovine Serum Albumin Sigma-Aldrich Cat# A3059 Paraformaldehyde Polysciences Cat# 00380-1 Intercalator-Ir Fluidigm Cat# 201192B EQ Four Element Calibration Beads Fluidigm Cat# 201078 Maxpar X8 Multimetal labeling kit Fluidigm Cat# 201300 Metal isotopes as chloride salts Fluidigm N/A Dimethyl Sulfoxide Sigma-Aldrich Cat# D8418 Cell-ID Cisplatin Fluidigm Cat# 201064 Metal isotopes as chloride salts Trace Sciences International N/A Protein Stabilizer PBS Candor Bioscience GmbH Cat# 131125 Critical Commercial Assays Test tube with 35 mm nylon mesh Corning Cat# 352235 Cardiometabolic panel Olink AB N/A Cell Regulation panel Olink AB N/A Cardiovascular II panel (CVD II) Olink AB N/A Cardiovascular III panel (CVD III) Olink AB N/A Development panel Olink AB N/A Immune Response panel Olink AB N/A Immuno-Oncology panel Olink AB N/A Oncology II panel Olink AB N/A Inflammation panel Olink AB N/A Metabolism panel Olink AB N/A Neurology, and Organ Damage panel Olink AB N/A Deposited Data FCS files, Mass cytometry This paper Protein expression data This paper Software and Algorithms Mass Cytometry Normalizer (Finck et al., 2013) https://github.com/nolanlab/bead-normalization/releases CyTOF software (v. 6.0.626) - https://www.fluidigm.com/ robCompositions 2.0.5 (Templ et al., 2011) https://cran.r-project.org/web/packages/robCompositions/ index.html Kohonen 3.0.2 (Wehrens and Buydens, 2007) https://cran.r-project.org/web/packages/kohonen/index.html dmbvs (Wadsworth et al., 2017) https://github.com/duncanwadsworth/dmbvs Princurve 2.1.3. (Hastie and Stuetzle, 1989) https://cran.r-project.org/web/packages/princurve/index.html MASS 7.3-51.1 (Venables and Ripley, 2002) https://cran.r-project.org/web/packages/MASS/index.html Sva 3.18.0 N/A http://bioconductor.org/packages/release/bioc/html/sva.html .. REAGENT or RESOURCE SOURCE IDENTIFIER Benzonase Sigma-Aldrich Cat# E1014 1X PBS Rockland Cat# MB-008 EDTA Rockland Cat# MB-014 Sodium Azide Sigma-Aldrich Cat# 71289 Bovine Serum Albumin Sigma-Aldrich Cat# A3059 Paraformaldehyde Polysciences Cat# 00380-1 Intercalator-Ir Fluidigm Cat# 201192B EQ Four Element Calibration Beads Fluidigm Cat# 201078 Maxpar X8 Multimetal labeling kit Fluidigm Cat# 201300 Metal isotopes as chloride salts Fluidigm N/A Dimethyl Sulfoxide Sigma-Aldrich Cat# D8418 Cell-ID Cisplatin Fluidigm Cat# 201064 Metal isotopes as chloride salts Trace Sciences International N/A Protein Stabilizer PBS Candor Bioscience GmbH Cat# 131125 Critical Commercial Assays Test tube with 35 mm nylon mesh Corning Cat# 352235 Cardiometabolic panel Olink AB N/A Cell Regulation panel Olink AB N/A Cardiovascular II panel (CVD II) Olink AB N/A Cardiovascular III panel (CVD III) Olink AB N/A Development panel Olink AB N/A Immune Response panel Olink AB N/A Immuno-Oncology panel Olink AB N/A Oncology II panel Olink AB N/A Inflammation panel Olink AB N/A Metabolism panel Olink AB N/A Neurology, and Organ Damage panel Olink AB N/A Deposited Data FCS files, Mass cytometry This paper Protein expression data This paper Software and Algorithms Mass Cytometry Normalizer (Finck et al., 2013) https://github.com/nolanlab/bead-normalization/releases CyTOF software (v. 6.0.626) - https://www.fluidigm.com/ robCompositions 2.0.5 (Templ et al., 2011) https://cran.r-project.org/web/packages/robCompositions/ index.html Kohonen 3.0.2 (Wehrens and Buydens, 2007) https://cran.r-project.org/web/packages/kohonen/index.html dmbvs (Wadsworth et al., 2017) https://github.com/duncanwadsworth/dmbvs Princurve 2.1.3. (Hastie and Stuetzle, 1989) https://cran.r-project.org/web/packages/princurve/index.html MASS 7.3-51.1 (Venables and Ripley, 2002) https://cran.r-project.org/web/packages/MASS/index.html Sva 3.18.0 N/A http://bioconductor.org/packages/release/bioc/html/sva.html ..

    Article Title: Molecular Signatures of Dengue Virus-Specific IL-10/IFN-γ Co-producing CD4 T Cells and Their Association with Dengue Disease
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Perforin, 175Lu Fluidigm Cat#3175004B; Clone B-D48 CCL3/CCL4, 176Yb R&D Systems Cat#MAB2701-100; Clone 93342; RRID: AB_2259652 Chemicals, Peptides, and Recombinant Proteins RPMI 1640 medium Omega Scientific, Inc. Cat#RP-21 Human serum Gemini Bio-Products Cat#100-512 GlutaMAX-I Thermo Fisher Scientific Cat#35050061 Penicillin and streptomycin Gemini Bio-Products Cat#400-109 DENV MP Grifoni et al., 2017; Weiskopf et al., 2016 N/A Brefeldin A (GolgiPlug) BD Biosciences Cat#51-2301KZ Cisplatin-194Pt Fluidigm Cat#201194 Intercalator-Ir Fluidigm Cat#201192B Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Critical Commercial Assays Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation Thermo Fisher Scientific Cat#11132D IFN-g Secretion Assay – Detection Kit (PE), human Miltenyi Biotech Cat# 130-054-202 IL-10 Secretion Assay – Detection Kit (APC), human Miltenyi Biotech Cat# 130-090-761 Deposited Data RNA-seq data This paper GEO: GSE132367; ImmPort: SDY 888 CyTOF data This paper ImmPort: SDY 888 Software and Algorithms FlowJo v10 FlowJo, LLC https://www.flowjo.com/ GraphPad Prism 8 GraphPad software https://www.graphpad.com/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Metascape Zhou et al., 2019 http://metascape.org/gp/index.html#/ main/step1 Cytobank Kotecha et al., 2010 https://www.cytobank.org/ MiXCR Bolotin et al., 2015, 2017 https://mixcr.readthedocs.io/en/master/ VDJtools Shugay et al., 2015 https://vdjtools-doc.readthedocs.io/ en/master/ ..

    Recombinant:



    Article Title: Molecular Signatures of Dengue Virus-Specific IL-10/IFN-γ Co-producing CD4 T Cells and Their Association with Dengue Disease
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Perforin, 175Lu Fluidigm Cat#3175004B; Clone B-D48 CCL3/CCL4, 176Yb R&D Systems Cat#MAB2701-100; Clone 93342; RRID: AB_2259652 Chemicals, Peptides, and Recombinant Proteins RPMI 1640 medium Omega Scientific, Inc. Cat#RP-21 Human serum Gemini Bio-Products Cat#100-512 GlutaMAX-I Thermo Fisher Scientific Cat#35050061 Penicillin and streptomycin Gemini Bio-Products Cat#400-109 DENV MP Grifoni et al., 2017; Weiskopf et al., 2016 N/A Brefeldin A (GolgiPlug) BD Biosciences Cat#51-2301KZ Cisplatin-194Pt Fluidigm Cat#201194 Intercalator-Ir Fluidigm Cat#201192B Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Critical Commercial Assays Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation Thermo Fisher Scientific Cat#11132D IFN-g Secretion Assay – Detection Kit (PE), human Miltenyi Biotech Cat# 130-054-202 IL-10 Secretion Assay – Detection Kit (APC), human Miltenyi Biotech Cat# 130-090-761 Deposited Data RNA-seq data This paper GEO: GSE132367; ImmPort: SDY 888 CyTOF data This paper ImmPort: SDY 888 Software and Algorithms FlowJo v10 FlowJo, LLC https://www.flowjo.com/ GraphPad Prism 8 GraphPad software https://www.graphpad.com/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Metascape Zhou et al., 2019 http://metascape.org/gp/index.html#/ main/step1 Cytobank Kotecha et al., 2010 https://www.cytobank.org/ MiXCR Bolotin et al., 2015, 2017 https://mixcr.readthedocs.io/en/master/ VDJtools Shugay et al., 2015 https://vdjtools-doc.readthedocs.io/ en/master/ ..

    Lysis:


    Protease Inhibitor:


    Western Blot:


    Chromatin Immunoprecipitation:


    SYBR Green Assay:


    Cell Isolation:


    Viability Assay:


    RNA Sequencing:


    Article Title: Molecular Signatures of Dengue Virus-Specific IL-10/IFN-γ Co-producing CD4 T Cells and Their Association with Dengue Disease
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Perforin, 175Lu Fluidigm Cat#3175004B; Clone B-D48 CCL3/CCL4, 176Yb R&D Systems Cat#MAB2701-100; Clone 93342; RRID: AB_2259652 Chemicals, Peptides, and Recombinant Proteins RPMI 1640 medium Omega Scientific, Inc. Cat#RP-21 Human serum Gemini Bio-Products Cat#100-512 GlutaMAX-I Thermo Fisher Scientific Cat#35050061 Penicillin and streptomycin Gemini Bio-Products Cat#400-109 DENV MP Grifoni et al., 2017; Weiskopf et al., 2016 N/A Brefeldin A (GolgiPlug) BD Biosciences Cat#51-2301KZ Cisplatin-194Pt Fluidigm Cat#201194 Intercalator-Ir Fluidigm Cat#201192B Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Critical Commercial Assays Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation Thermo Fisher Scientific Cat#11132D IFN-g Secretion Assay – Detection Kit (PE), human Miltenyi Biotech Cat# 130-054-202 IL-10 Secretion Assay – Detection Kit (APC), human Miltenyi Biotech Cat# 130-090-761 Deposited Data RNA-seq data This paper GEO: GSE132367; ImmPort: SDY 888 CyTOF data This paper ImmPort: SDY 888 Software and Algorithms FlowJo v10 FlowJo, LLC https://www.flowjo.com/ GraphPad Prism 8 GraphPad software https://www.graphpad.com/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Metascape Zhou et al., 2019 http://metascape.org/gp/index.html#/ main/step1 Cytobank Kotecha et al., 2010 https://www.cytobank.org/ MiXCR Bolotin et al., 2015, 2017 https://mixcr.readthedocs.io/en/master/ VDJtools Shugay et al., 2015 https://vdjtools-doc.readthedocs.io/ en/master/ ..

    Control:


    CRISPR:


    Virus:


    Staining:


    Article Title: Molecular Signatures of Dengue Virus-Specific IL-10/IFN-γ Co-producing CD4 T Cells and Their Association with Dengue Disease
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Perforin, 175Lu Fluidigm Cat#3175004B; Clone B-D48 CCL3/CCL4, 176Yb R&D Systems Cat#MAB2701-100; Clone 93342; RRID: AB_2259652 Chemicals, Peptides, and Recombinant Proteins RPMI 1640 medium Omega Scientific, Inc. Cat#RP-21 Human serum Gemini Bio-Products Cat#100-512 GlutaMAX-I Thermo Fisher Scientific Cat#35050061 Penicillin and streptomycin Gemini Bio-Products Cat#400-109 DENV MP Grifoni et al., 2017; Weiskopf et al., 2016 N/A Brefeldin A (GolgiPlug) BD Biosciences Cat#51-2301KZ Cisplatin-194Pt Fluidigm Cat#201194 Intercalator-Ir Fluidigm Cat#201192B Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Critical Commercial Assays Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation Thermo Fisher Scientific Cat#11132D IFN-g Secretion Assay – Detection Kit (PE), human Miltenyi Biotech Cat# 130-054-202 IL-10 Secretion Assay – Detection Kit (APC), human Miltenyi Biotech Cat# 130-090-761 Deposited Data RNA-seq data This paper GEO: GSE132367; ImmPort: SDY 888 CyTOF data This paper ImmPort: SDY 888 Software and Algorithms FlowJo v10 FlowJo, LLC https://www.flowjo.com/ GraphPad Prism 8 GraphPad software https://www.graphpad.com/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Metascape Zhou et al., 2019 http://metascape.org/gp/index.html#/ main/step1 Cytobank Kotecha et al., 2010 https://www.cytobank.org/ MiXCR Bolotin et al., 2015, 2017 https://mixcr.readthedocs.io/en/master/ VDJtools Shugay et al., 2015 https://vdjtools-doc.readthedocs.io/ en/master/ ..

    Fluorescence In Situ Hybridization:


    Hybridization:


    Activation Assay:

    Article Title: Molecular Signatures of Dengue Virus-Specific IL-10/IFN-γ Co-producing CD4 T Cells and Their Association with Dengue Disease
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Perforin, 175Lu Fluidigm Cat#3175004B; Clone B-D48 CCL3/CCL4, 176Yb R&D Systems Cat#MAB2701-100; Clone 93342; RRID: AB_2259652 Chemicals, Peptides, and Recombinant Proteins RPMI 1640 medium Omega Scientific, Inc. Cat#RP-21 Human serum Gemini Bio-Products Cat#100-512 GlutaMAX-I Thermo Fisher Scientific Cat#35050061 Penicillin and streptomycin Gemini Bio-Products Cat#400-109 DENV MP Grifoni et al., 2017; Weiskopf et al., 2016 N/A Brefeldin A (GolgiPlug) BD Biosciences Cat#51-2301KZ Cisplatin-194Pt Fluidigm Cat#201194 Intercalator-Ir Fluidigm Cat#201192B Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat#00-5523-00 Critical Commercial Assays Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation Thermo Fisher Scientific Cat#11132D IFN-g Secretion Assay – Detection Kit (PE), human Miltenyi Biotech Cat# 130-054-202 IL-10 Secretion Assay – Detection Kit (APC), human Miltenyi Biotech Cat# 130-090-761 Deposited Data RNA-seq data This paper GEO: GSE132367; ImmPort: SDY 888 CyTOF data This paper ImmPort: SDY 888 Software and Algorithms FlowJo v10 FlowJo, LLC https://www.flowjo.com/ GraphPad Prism 8 GraphPad software https://www.graphpad.com/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Metascape Zhou et al., 2019 http://metascape.org/gp/index.html#/ main/step1 Cytobank Kotecha et al., 2010 https://www.cytobank.org/ MiXCR Bolotin et al., 2015, 2017 https://mixcr.readthedocs.io/en/master/ VDJtools Shugay et al., 2015 https://vdjtools-doc.readthedocs.io/ en/master/ ..



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